掲載種別：研究論文（学術雑誌）   出版者・発行元：Institute of Electrical and Electronics Engineers (IEEE)  

DOI： 10.1109/jstqe.2021.3064406

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：Institute of Electrical and Electronics Engineers (IEEE)  

Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.

DOI： 10.1109/jstqe.2020.3038403

掲載種別：研究論文（その他学術会議資料等）  

Sound is an important invisible physical phenomenon that needs to be explained in several physical and biological processes, along with visual phenomena. For this purpose, multiparameter digital holography (DH) has been proposed to visualize both features simultaneously due to the phase and amplitude reconstruction properties of DH. In this paper, we present a brief review on sound field imaging techniques with special focus on the multiparameter imaging capability of DH for visualizing sound and visual features. The basic theory and several experimental results with very high-speed recordings are also presented to demonstrate sound field imaging for the audible range aswell as in the ultrasound range.

DOI： 10.1364/AO.415162

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Association for the Advancement of Science (AAAS)  

Sustained neuropathic pain from injury or inflammation remains a major burden for society. Rodent pain models have informed some cellular mechanisms increasing neuronal excitability within the spinal cord and primary somatosensory cortex (S1), but how activity patterns within these circuits change during pain remains unclear. We have applied multiphoton in vivo imaging and holographic stimulation to examine single S1 neuron activity patterns and connectivity during sustained pain. Following pain induction, there is an increase in synchronized neuronal activity and connectivity within S1, indicating the formation of pain circuits. Artificially increasing neuronal activity and synchrony using DREADDs reduced pain thresholds. The expression of N-type voltage-dependent Ca²⁺ channel subunits in S1 was increased after pain induction, and locally blocking these channels reduced both the synchrony and allodynia associated with inflammatory pain. Targeting these S1 pain circuits, via inhibiting N-type Ca²⁺ channels or other approaches, may provide ways to reduce inflammatory pain.

DOI： 10.1126/sciadv.abd8261

掲載種別：研究論文（学術雑誌）   出版者・発行元：The Optical Society  

DOI： 10.1364/ao.404208

その他リンク： https://www.osapublishing.org/viewmedia.cfm?URI=ao-60-4-A195&seq=0

掲載種別：研究論文（学術雑誌）  

© 2020 Elsevier Ltd The sound wave has been recorded and visualized by optical means such as digital holography (DH), where light wave carries the sound information. However, in DH, an additional reference beam is required, which can create problems in the applications where long-distance propagations are required for sound field studies. In this work, we present a new way of sound wave recording and visualization by a common-path DH in which a single light beam can carry the sound information and near the recording device, this single light beam is divided into the object and reference beams by using a beam splitter. One of the beams is filtered to generate a clean reference beam. Therefore, this configuration of DH eliminates the need of a reference beam from the starting point and makes the system simpler, compact, and stable, in comparison to the optical sound wave recording setup of traditional DH. The presented optical system of sound wave recording is suitable for applications where the single beam is required to observe invisible sound phenomena at far position. We present experimental results of vibration frequency and voice information visualization to demonstrate the proposed system.

DOI： 10.1016/j.optlaseng.2020.106331

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：IOP Publishing  

DOI： 10.1088/2040-8986/abb3a4

その他リンク： https://iopscience.iop.org/article/10.1088/2040-8986/abb3a4/pdf

掲載種別：研究論文（学術雑誌）  

A single-shot common-path off-axis self-interference dual-wavelength digital holographic microscopic (DHM) system based on a cube beam splitter is demonstrated to expand the phase range in a stepped microstructure and for simultaneous measurement of the refractive index and physical thickness of a specimen. In the system, two laser beams with wavelengths of 532 nm and 632.8 nm are used. These laser beams are combined to transilluminate the object under study, then the object beam is divided into two beams by using a beam splitter oriented in such a way that both the beams propagate in almost the same direction, with an appropriate lateral separation between them. One of the object beams is spatially filtered at its Fourier plane, using a pinhole to generate a reference spherical beam free from the object information. The reference beam interferes with the object beam to form a digital hologram at the faceplate of the image sensor. The phase information is extracted from a single recorded digital hologram using the phase aberration compensation method that is based on principal component analysis (PCA). Owing to the common-path configuration, the system shows high temporal phase stability and it is less vibration-sensitive compared to counterparts such as a Mach–Zehnder type DHM. The performance of the dual-wavelength DHM system is verified in two different application fields by conducting the experiments using microsphere beads and living plant cells.

DOI： 10.1364/AO.395001

掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media {LLC}  

DOI： 10.1038/s41598-020-64028-x

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：{SPIE}-Intl Soc Optical Eng  

DOI： 10.1117/1.jbo.25.3.032010

掲載種別：研究論文（学術雑誌）   出版者・発行元：SPIE-Intl Soc Optical Eng  

We propose a nonscanning three-dimensional (3-D) fluorescence imaging technique using the transport of intensity equation (TIE) and free-space Fresnel propagation. In this imaging technique, a phase distribution corresponding to defocused fluorescence images with a point-light-source-like shape is retrieved by a TIE-based phase retrieval algorithm. From the obtained phase distribution, and its corresponding amplitude distribution, of the defocused fluorescence image, various images at different distances can be reconstructed at the desired plane after Fresnel propagation of the complex wave function. Through the proposed imaging approach, the 3-D fluorescence imaging can be performed in multiple planes. The fluorescence intensity images are captured with the help of an electrically tunable lens; hence, the imaging technique is free from motion artifacts. We present experimental results corresponding to microbeads and a biological sample to demonstrate the proposed 3-D fluorescence imaging technique.

DOI： 10.1117/1.jbo.25.3.032004

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：レーザー学会  

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：The Optical Society  

A new type of functional optical microscope system called three-dimensional (3D) stimulation and imaging-based functional optical microscopy (SIFOM) is proposed, to the best of our knowledge. SIFOM can precisely stimulate user-defined targeted biological cells and can simultaneously record the volumetric fluorescence distribution in a single acquisition. Precise and simultaneous stimulation of fluorescent-labeled biological cells is achieved by multiple 3D spots generated by digital holograms displayed on a phase-mode spatial light modulator. Single-shot 3D acquisition of the fluorescence distribution is accomplished by common-path off-axis incoherent digital holographic microscopy in which a diffraction grating with a focusing lens is displayed on another phase-mode spatial light modulator. The effectiveness of the proposed functional microscope system was verified in experiments using fluorescent microbeads and human lung cancer cells located at various defocused positions. The system can be used for manipulating the states of cells in optogenetics.

DOI： 10.1364/ol.43.005447

その他リンク： https://www.osapublishing.org/viewmedia.cfm?URI=ol-43-21-5447&seq=0

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Oxford University Press (OUP)  

In this review, we introduce digital holographic techniques and recent progress in multidimensional sensing by using digital holography. Digital holography is an interferometric imaging technique that does not require an imaging lens and can be used to perform simultaneous imaging of multidimensional information, such as three-dimensional structure, dynamics, quantitative phase, multiple wavelengths and polarization state of light. The technique can also obtain a holographic image of nonlinear light and a three-dimensional image of incoherent light with a single-shot exposure. The holographic recording ability of this technique has enabled a variety of applications.

DOI： 10.1093/jmicro/dfy007

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OPTICAL SOC AMER  

A technique of three-dimensional (3D) intensity retrieval from defocused, two-dimensional (2D) fluorescent images in the multimodal digital holographic microscopy (DHM) is proposed. In the multimodal DHM, 3D phase and 2D fluorescence distributions are obtained simultaneously by an integrated system of an off-axis DHM and a conventional epifluorescence microscopy, respectively. This gives us more information of the target; however, defocused fluorescent images are observed due to the short depth of field. In this Letter, we propose a method to recover the defocused images based on the phase compensation and backpropagation from the defocused plane to the focused plane using the distance information that is obtained from a 3D phase distribution. By applying Zernike polynomial phase correction, we brought back the fluorescence intensity to the focused imaging planes. The experimental demonstration using fluorescent beads is presented, and the expected applications are suggested. (C) 2017 Optical Society of America

DOI： 10.1364/OL.42.001796

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   出版者・発行元：The Optical Society  

DOI： 10.1364/ol.42.000383

記述言語：日本語   掲載種別：研究論文（その他学術会議資料等）   出版者・発行元：アドコム・メディア  

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：OPTICAL SOC JAPAN  

Hybrid digital holographic microscopy that combines fluorescence microscopy and digital holographic microscopy into a single system for biological applications is proposed. In the proposed system, a phase image and a fluorescence image can be obtained simultaneously by selecting the different wavelengths of the fluorescent light and the phase measurement. Especially for biological applications, the cell structure can be obtained by the phase imaging based on digital holography and nucleus of the cell can be obtained by the fluorescence imaging. The measurement of fluorescence beads and egera densa presented the feasibility of simultaneous detection of both a phase image and a fluorescence image.

DOI： 10.1007/s10043-015-0042-4

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Indian Spectroscopy Society  

掲載種別：記事・総説・解説・論説等（学術雑誌）  

In this paper, we integrate the fluorescence and phase imaging simultaneously that uses the light field and the transport of intensity equation (TIE). Here, the fluorescence information is obtained by the light field imaging with an electrically tunable lens while phase imaging is done by TIE. For fluorescence, different intensity stacks are captured with image sensor to be used for reconstructing the fluorescence images using the concept of light field. To get the phase information of the same sample along with fluorescence, the three intensity images are captured by another image sensor for solving TIE for phase data. Experimental results of micro beads are presented in support of simultaneous imaging technique.

DOI： 10.1117/12.2615674

掲載種別：記事・総説・解説・論説等（学術雑誌）  

We have proposed a new configuration of the single-shot, dual-wavelength, common-path, off-axis DHM system. The proposed system is used for decoupling the refractive index and thickness of a biological specimen.

DOI： 10.1117/12.2586317

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：The Optical Society  

A method for single pixel imaging suitable for high definition imaging is demonstrated. The experimental system consists of a laser with two dimensional scanning, a digital micro-mirror device, a condenser lens, and a photo detector. It is shown that the system can output an image with 1024×1024 pixels.

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. In this research, we have proposed a multimodal fast fluorescent imaging by an electrical tunable lens, and quantitative phase imaging by digital holography. In conventional confocal microscopy, sectioning in z direction is realized either by moving objective lens or sample stage. In order to speed up the imaging, and utilize in the specific applications such as light stimulation system in optogenetics, the sectioning is realized by changing the focal power of an electrical tunable lens. The preliminary experiment using fluorescence beads and its relation between focal power and depth change is shown.

DOI： 10.1117/12.2573299

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2020 SPIE. Downloading of the abstract is permitted for personal use only. Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

DOI： 10.1117/12.2559794

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：The Optical Society  

We present fluorescence imaging of plant cells based on transport of intensity equation and Fresnel propagation. At first, the phase distribution is obtained from the recorded three-defocus fluorescence intensity images and then from phase and corresponding intensity defocus image, the focus images of plant cell sample can be retrieved at various planes after free space propagation.

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：The Optical Society  

A highly stable multimodal 3D imaging system is developed, for simultaneous recording and retrieval of 3D fluorescence imaging and quantitative phase distribution of a biological sample, by incorporating the single-shot common-path off-axis configurations of the incoherent and coherent digital holographic microscopes, respectively.

掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：SPIE  

DOI： 10.1117/12.2522557

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© OSA 2019 © 2019 The Author(s) We demonstrate a common-path off-axis incoherent digital holographic and active multi-beam illumination microscopic system for 3D fluorescence imaging. The proposed system is employed for the 3D imaging of the fluorescent beads and plant Physcomitrella patens.

DOI： 10.1364/DH.2019.Th1A.7

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：SPIE  

Binary-phase-only reconstruction for holographic 3D display has a problem that degrades the image quality by enhancing the speckle. Image segmentation method is applied to reduce the speckle effect. Numerical and experimental results are presented to verify the proposed method.

DOI： 10.1117/12.2540345

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2019 SPIE. Multispots in three-dimensions generated by phase mode spatial light modulators (SLMs) are very useful in laser processing or light induced biological treatments, such as optogenetics. So far, the intensity of the focus spots varies depending to the distance from optical axis. In order to equalize intensities in all generated spots, simple feedback method was applied. Further, look up table was created by dividing imaging area in sectors. Experiment results show improvement in the random spots at some degree, however, further analyzation is required.

掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：DHIP2018  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：WIO2018  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：OSA2018  

掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：SPIE  

DOI： 10.1117/12.2302683

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2018 SPIE. In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

DOI： 10.1117/12.2501202

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2018 SPIE. This paper introduces a new type of multimodal digital holographic microscopy for biological applications. Off-axis digital holography is applied both in 3D phase imaging and 3D fluorescence imaging. In addition, image recovery by iterative method to obtain focused fluorescence images are introduced.

DOI： 10.1117/12.2321988

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：OSA - The Optical Society  

Digital phase conjugation is applied to improve the focusing property in a weakly scattering medium for achieving high-resolution OCT. The distorted phase distribution is measured by the guided laser light and then the phase modulation of a low-coherence light for OCT is generated to compensate the distorted phase as well as wavelength conversion. Experimental results for improving the focusing property are presented.

DOI： 10.1364/3D.2018.3W5G.3

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2017 IEEE. Multimodal imaging in biology plays an important role. So far the 3D visualization of fluorescence is realized by scanning systems. For faster imaging, the sectioning process intrinsically exhibits limitations in recording speed. We presented common-path off-axis incoherent digital holography using dual gratings on dual Fresnel lenses. Using the proposed method, single-shot, 3D fluorescence imaging is realized. We also suggested an integrated system of 3D fluorescence and quantitative phase imaging. Preliminary results on off-axis incoherent digital holography and multimodal imaging are demonstrated.

DOI： 10.1109/WIO.2017.8038183

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC  

Digital holography provides a method of the 3-D recording and numerical reconstruction by a simple optical system and a computer. Quantitative measurement and numerical refocusing are major characteristics. So far, many physical parameters such as amplitude, phase, polarization, fluorescence, and spectra can be obtained independently. Recently, multimodal imaging that can obtain simultaneously two or more physical parameters by combining digital holographic microscope and other optical microscopes such as a fluorescence optical microscope and a Raman scattering microscope has emerged. In this review, physical parametric imaging techniques based on digital holography are presented and then these techniques are enhanced to develop multimodal imaging based on digital holography.

DOI： 10.1109/JPROC.2017.2656148

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：SPIE-INT SOC OPTICAL ENGINEERING  

A visualization technique of sound wave propagation using digital holography with heterodyne technique is presented. In the proposed method, the frequency of the interference pattern in an off-axis digital holography is down converted into the detectable frequency in an image sensor operated at the video frame rate by using the heterodyne interferometer. We present the principle of the recording technique and experimental results are described.

DOI： 10.1117/12.2270101

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

DOI： 10.1117/12.2264217

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：SPIE  

This paper provides overall analytical insights on the common-path incoherent digital holography using dual-focusing lens with diffraction gratings. Especially when one tries to seek an off-Axis solution using the suggested configuration, the low temporal and spatial coherence require specific conditions on parameters of the set-up. AA mathematical explanation on the off-Axis digital holography is described.

DOI： 10.1117/12.2275019

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© OSA 2017. Fluorescent images that are blurred by large focus displacements are corrected by phase compensation. This is possible when the defocusing distance is obtained from an external measurement. A multi-modal digital holography that enables both quantitative phase imaging and 2D fluorescent imaging provides complimentary information from the same measuring object, such as living cells with fluorescent labels. In this paper, we adapted Zernike polynomials to correct the defocused fluorescent images, and used coefficients that are linear to the defocusing distances. Experimental results from bead images are presented.

DOI： 10.1364/DH.2017.Tu2A.6

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

DOI： 10.1117/12.2264427

掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：IEEE  

© 2016 IEEE. Multi-modal 3D imaging based on digital holography is reviewed. In the developed system, 3D phase and 2D fluorescence distributions can be measured simultaneously. This characteristic can be used for measurement of live cell activity. We present the preliminary experimental results. One is the observation of microbeads embedded by fluorescence molecules. Second is the observation of plant cell.

DOI： 10.1109/WIO.2016.7745587

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2016 SPIE. For defect detection or undesired object in commercial products, it is required to develop a fast measurement system that can obtain three-dimensional distribution of surface of the opaque medium such as metal or inside of the transparent medium. For this purpose, we fabricated a digital holographic microscope using a fast image sensor when the phase object is put on a fast movable stage. In the fabricated system, an image sensor operated at maximum frame rate of 2000 fps and a movable stage operated at maximum speed of 300 mm/s are introduced. Under the continuous wave illumination, motion-blurred phase object is reconstructed. By using numerical processing such as deconvolution filter, the reconstructed phase distribution is much improved. Numerical results are presented.

DOI： 10.1117/12.2216745

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：IEEE  

Fluorescent imaging and phase contrast imaging are two major techniques for live cell studies. One can obtain those two types of images by switching illumination and filter sets, and this process usually consumes unnecessary time. In order to image dynamical cell behaviors, we have presented a multi- modal digital holographic microscopic method to obtain both quantitative phase images and fluorescent images at the same time. We used Physcomitrella patens the moss to test the proposed system. Two-dimensional images of chloroplasts and quantitative phase information were obtained simultaneously under continuous exposure of laser light. We observed shrinkage in cell size and concentration in the chloroplasts distribution.

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2016 SPIE. One of fast measurement systems of μm-size phase objects based on digital holographic microscope with transmission geometry is presented. For building a 3D inspection system of the phase objects, the improvement of recovered phase image is discussed. Under the CW laser illumination, the movement afterimage of phase object was observed. The phase object is recovered by deconvolution filter. Experimental and numerical evaluation are presented.

DOI： 10.1117/12.2229727

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2016 SPIE. Multi-modal digital holographic microscopy is a combination of epifluorescence microscopy and digital holographic microscopy, the main function of which is to obtain images from fluorescence intensity and quantified phase contrasts, simultaneously. The proposed system is mostly beneficial to biological studies, with the reason that often the studies are depending on fluorescent labeling techniques to detect certain intracellular molecules, while phase information reflecting properties of unstained transparent elements. This paper is presenting our latest researches on applications such as randomly moving micro-fluorescent beads and living cells of Physcomitrella patens. The experiments are succeeded on obtaining a succession of wide-field fluorescent images and holograms from micro-beads, and different depths focusing is realized via numerical reconstruction. Living cells of Physcomitrella patens are recorded in the static manner, the reconstruction distance indicates thickness of cellular structure. These results are implementing practical applications toward many biomedical science researches.

DOI： 10.1117/12.2212086

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：Institute of Electrical and Electronics Engineers Inc.  

Reconstructed position of the optical absorber embedded in a homogeneous scattering medium is evaluated. In the numerical reconstruction, the ratio of the intensity distribution in the scattering medium with an absorber to that in the scattering medium without the absorber is used.

DOI： 10.1109/SICE.2015.7285398

掲載種別：記事・総説・解説・論説等（学術雑誌）  

© 2015 IEEE. Fast measurement system of a moving three-dimensional phase object using digital holographic microscopy is presented. In the fabricated system, a three-dimensional phase object is put on a fast movable stage. Under the CW laser illumination, the holograms of the phase object are superimposed. We experimentally investigated the effect of the speed of the movable stage on the reconstructed data.

DOI： 10.1109/WIO.2015.7206914

その他リンク： http://orcid.org/0000-0002-6366-9196

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：SPIE-INT SOC OPTICAL ENGINEERING  

It will be useful in biological applications if two or more physical parameters are simultaneously measured in a digital holographic microscope. In this paper, we present a hybrid digital holographic microscope that can measure simultaneously three-dimensional (3D) phase and 3D fluorescence distributions. This property has big advantage compared with conventional optical microscopes such as phase contrast microscope and fluorescence microscope. We present an optical setup for measuring both phase and fluorescence images. In the experiments, two objects that are fluorescence beads and egera densa are used. The separation method of phase and fluorescence images is presented.

DOI： 10.1117/12.2185665

その他リンク： http://orcid.org/0000-0001-9767-0059

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：IEEE  

A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and fluorescence images is proposed. The proposed microscopic system is designed by combination of a transmission type off-axis digital holographic configuration and a reflection type Fresnel incoherent holographic configuration, aimed for biological application. Owing to the single acquisition, signal processing which separates a superposed hologram is proposed.

掲載種別：記事・総説・解説・論説等（学術雑誌）  

その他リンク： http://orcid.org/0000-0002-6366-9196

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：DHIP実行委員会  

記述言語：日本語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：日本光学会（応用物理学会）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）  

記述言語：英語   会議種別：口頭発表（一般）  

開催地：Delhi, New Delhi  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Delhi, New Delhi  

記述言語：日本語   会議種別：口頭発表（招待・特別）  

開催地：筑波大学東京キャンパス文京校舎,東京  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：筑波大学東京キャンパス文京校舎,東京  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：筑波大学東京キャンパス文京校舎,東京  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：筑波大学東京キャンパス文京校舎,東京  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Suzhou; China;  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：名古屋国際会議場，愛知県  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Beijing; China  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Taipei;Taiwan  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Taipei;Taiwan  

記述言語：英語   会議種別：ポスター発表  

開催地：Taipei;Taiwan  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：東京都  

記述言語：英語   会議種別：口頭発表（一般）  

開催地：オーランド  

記述言語：英語   会議種別：口頭発表（一般）  

開催地：中国、上海  

記述言語：英語   会議種別：口頭発表（一般）  

開催地：神奈川、横浜  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：神奈川、横浜  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：東京、日本  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：静岡、日本  

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Pattaya city; Thailand  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：東京、日本